We value random discovery, serendipity, and power of observation. At the same time, we pursue systematic studies using a variety of methodologies, from serum biochemistry, to in vivo pharmacology to population studies in healthy persons and in patients. We are always looking for enthusiastic and energetic individuals.

  
Wang_GuankuiGuankui Wang, PhD, Postdoctoral Associate
Guankui Wang received a bachelor’s degree in Animal Science from Northwest Agriculture and Forestry University of China in 2007. He completed his PhD in Animal Physiology from the University of Idaho in 2012. After one-year postdoctoral training in the Department of Chemistry and Biochemistry at the University of Colorado Boulder, he joined the Simberg Lab as a Postdoctoral Fellow in November 2013. He has been working on multiple projects on the development of iron oxide nanoparticles for targeted drug delivery and magnetic resonance imaging, the interactions of nanoparticles with the immune system, the isolation of circulating tumor cells from cancer patients’ blood, and cell-based therapy for leukemia.
ernest-gromanErnest Groman, PhD
Dr. Groman, possibly the oldest postdoc on campus, earned a BS in mathematics from the University of North Carolina-Chapel Hill and a PhD in biochemistry from the University of California-Berkeley. After postdoctoral studies at Massachusetts General Hospital and Harvard Medical School, he cofounded Advanced Magnetics and BioPhysics Assay Laboratory. He is the lead inventor of Feraheme, a drug used to treat iron deficiency anemia and as an experimental MRI contrast agent to study inflammation (following chemotherapy), vascular disease and lymphatic involvement in various cancers. His interests include translation of grant funded projects into products, drug delivery using nanoparticles, cell labeling with nanoparticles, development of dual label contrast agents and applications of magnetic fields to improve drug delivery.
hanmant-gaikwadHanman Gaikwad, PhD
Hanmant Gaikwad received a Master degree (M.Sc.) in Organic Chemistry from the Shivaji University, Kolhapur in 2005. He completed his PhD in Organic and Medicinal Chemistry from the CSIR-Indian Institute of Chemical Technology (IICT), Hyderabad, India in 2013. Later, in 2013, he joined as a Post-doctoral researcher in Dr. Galia Blum's research group at School of Pharmacy, Hebrew University, Jerusalem. He worked on molecular imaging of cancer using X‑ray computed tomography with protease targeted iodinated activity-based probes. Then he joined the Simberg Lab as a postdoctoral Associate in December 2017. His research work focuses on development of new class of small molecules, nanoparticle conjugates, lipid conjugates and antibody conjugates with cleavable linkers for targeted drug delivery and molecular imaging.
laren-lofchyLaren Lofchy, PhD Candidate
Laren Lofchy, graduate student. She received her Bachelors’ of Science and Engineering (BSE) in Chemical Engineering from the University of Michigan in 2017 and joined the Simberg lab as a PhD student soon after. She is currently researching the mechanism of nanoparticle extravasation with the hope of one day exploiting it. If she had free time, she’d be found skiing, backpacking, or composing experimental electronic music.
geoffrey-giffordGeoffrey Gifford, BSc, Professional Research Assistant
Fanfang-ChenFangfang Chen, PhD, Visiting Research Professor (Jilin University, China)
chunyan-yangChunyan Yang, MD, PhD, Visiting Scholar
Dr. Yang was born in February 1980. She got the Master’s degree in Internal Medicine of Jilin University in June 2008 and the Doctor's degree of Jilin University in June 2012. Since August 2012, she has been an attending physician in the department of cardiology of China-Japan Union Hospital of Jilin University.

She likes reading newspapers, magazines and works of literature, etc. By doing so, she learns about the wonderful world and enriches her spiritual world.
ping-renPing Ren, MD, PhD, Visiting Scholar
Dr. Ren is a practicing surgeon in the Department of Thoracic Surgery, the first hospital of Jilin University. He received his PhD and MD from Jilin University.
  
paulina-doPaulina Do, PharmD Candidate
weston-smithWeston Smith, PhD, Postdoctoral Associate
vivian-vuVivian Vu, Professional Research Assistant
huy-tranHuy Tran, Undergraduate Bioengineering Program, CU Boulder
James-GriffinJames Griffin, BSc., Research Associate
abi-prabhakaranAbilash (Abi) Prabhakaran, Cherry Creek High School Student
BarbaraBrennemanBarbara Brenneman, BSc, PharmD Candidate
JessicaJonesJessica Jones, BSc, PharmD Candidate
HalliBenasuttiHalli Benasutti, BSc, Research Assistant
HyeHyunKimHye Hyun (Sally) Kim, undergraduate student
Chemical and Biological Engineering
University of Colorado Boulder
swetha-inturiSwetha Inturi, PhD, Postdoctoral Associate
christopher-bullChristopher Bull, PharmD Candidate Student
Mukthavaram_RajeshRajesh Mukthavaram, PhD, Postdoctoral Scholar
Shi_GuixinGuixin Shi, PhD, Postdoctoral Scholar
Cui_WenjunWenjin Cui, PhD, Postdoctoral Scholar
Kim_AmoseAmose Kim, BS, Research Assistant
Cheng_KevinKevin Cheng, BSc, Research Assistant
Mittal_RitishRitish Mittal, BSc, Research Assistant
Restrepo_LauraLaura Restrepo, MSc, Research Assistant

GUAVA high throughput flow cytometer with 2 lasers/6 channels

GUAVA-Cytometer

Zeiss Axio Observer 5 epifluorescent microscope equipped with 5 filter cubes and Exelitas near infrared light source and NIR filter cube (760nm excitation/780nm emission)

Zeiss-Axio-Observer

Biosafety cabinet for patient samples

Biosafety-Cabinet

Thermomixers

Thermomixers

 

Refrigerated tabletop centrifuge

Refrigerated-tabletop-centrifuge

Biosafety hood for cell culture

Biosafety-hood

Agilent 1200 HPLC with UV-Vis detector

Agilent-1200

Beckman Optima ultracentrifuge

Beckman-Optima-ultracentrifuge

Lyophilizer

Lyophilizer

Spectrophotometer

Spectrophotometer

Fiber optical near-infrared spectroscopy (FONIRS) for in vivo near infrared spectroscopy

FONIRS

Olympus IX73 Inverted Light Microscope

Olympus-IX73

PCR and gel electrophoresis

PCR-and-gel-electrophoresis

PALL reverse osmosis ultrafiltration system

PALL-Reverse-Osmosis-Ultrafiltration-System

Isoflurane anesthesia machine

Isoflurane-Anesthesia-Machine

Automated cell counter

Automated-cell-counter

 

Cell culture centrifuge

Cell-culture-centrifuge

Cell incubator

Cell-incubator

Rotary evaporator

Rotary-evaporator

Cytospin

Cytospin

Chemical hood

Chemical-hood

QSonica 500 probe sonicator

QSonica-500

Harvard Apparatus PS-1 ex vivo perfusion system with precise control of organ humidity, pulse rate, flow rate, pressure, temperature and oxygenation

Harvard-Apparatus

ISOLERA flash chromatography system

ISOLERA-flash-chromatography-system

  • Clean up after yourself and maintain a clean and orderly lab space.
  • Safety is very important: wear goggles, a coat, and gloves, and work in the hood whenever necessary.
  • Dispose of solvents and chemicals in the bottle in the hood; never into the sink!
  • Work with volatile solvents in the hood only.
  • All reagents should be put back in their designated place after use.
  • If running low on a reagent, please order it before it runs out.
  • Write everything you do in the lab book and sign and date it each day. Never omit any details – even the small ones!
  • Document experiments with images and quantitative data; you can include attachments to the lab book, including printouts and gels. Summarize the experiment, including method, result and conclusion, preferably in PowerPoint.
  • Each experiment gets the following: year-month-day-initials-description, for example.

Advice for Scientists

  • If there is a problem, it is OK to admit it and ask for help.
  • Always try to quantify your observations. Pictures are nice to publish, but only quantitative data can give you confidence in the results.
  • Always try to include positive and negative controls.
  • The experiments should be run at sufficient number of replicates (3 or more) and repeated for statistical power. You must be sure that the result is correct!
  • Stay focused on the project. Be persistent: don't give up easily
  • 10-20% of time should be improvisation and innovation; Try crazy things, and don't be afraid to make mistakes!
  • Read literature periodically, brainstorm ideas, go to seminars and conferences, listen to news – you never know where new ideas can come from!
  • It is OK to argue and defend your point of view.
  • If you don't have time to do it right, do you think you will have time to do it over?
  • "Never leave that till tomorrow which you can do today." - Benjamin Franklin

We are a green lab!

We believe that whatever we do in the lab has an environmental price. We do science, which is a cool thing, and it benefits humankind. However, science can take a serious environmental toll. For example, each chemical hood uses as much energy as the average American household. The chemicals that we order and synthesize in the lab also use a lot of energy to make them. And chemicals that aren’t discarded properly can pollute water sources and kill marine life.

So, in our lab, we strive to be sustainable and have embraced the Reduce, Reuse, and Recycle model to be an environmentally responsible as possible. Here are some common tips we use in our lab that you could use, too:

  • Reduce chemical use: Don't order a chemical before checking if someone else might actually have it. For this purpose, we catalogue all of our chemicals. Also, ask colleagues if you can borrow the chemical from them.
  • Reduce electricity use: After hours and whenever possible, turn off centrifuges, equipment, chemical and tissue hoods, and lights. Don't leave freezer and fridge doors open for an extended period of time.
  • Recycle: Never dispose of chemicals or solvents in the sink, including methanol-containing buffers; use special containers as specified by the safety department. Dispose recyclable items in the recycling bin.
  • Reuse: Try to buy used equipment whenever possible and reuse lab supplies as much as possible.