Aut​​horship

Co-authorship is generally expected when core personnel have made significant contributions to the research in the form of consultation, experimental design, method development, data analysis and/or data interpretation. Significant is defined as “the project would not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel”. In such cases, core personnel should have the opportunity to review and edit the appropriate sections of a manuscript before submission.

Acknowledgement of facility contributions is expected in publications that include any data generated in the facility. For example, when fee-for-service is performed with no method development and minimum effort by Core personnel. An example of an appropriate acknowledgment is “The authors wish to thank the University of Colorado School of Pharmacy, Mass Spectrometry Facility for analyzing samples”.

Please inform us when relevant publications are accepted and forward us a copy for our records. We may also post citation information on our website. This information is vital to the continued support of the facility.

Grant Assista​nce

We are very happy to provide letters of support and assistance in grant writing. For letters of support, please allow at least 2 weeks from the time of your request. Please furnish the following information: Title of grant, agency to which you are submitting the grant, your title and mailing address, and 1-3 sentences on the goals (or specific aims) of your proposal.

Our facility is also dependent on grant support for long-term success and we appreciate your support in keeping the facility funded. To determine if percent effort by core personnel is required, please consider the following:

  1. Does the proposal include a specific aim(s) that focuses on mass spectrometry?
  2. Is method development is required?
  3. Are there large numbers of samples are to be ​analyzed?
  4. Would the aim or proposal not receive a favorable review without the support of Core personnel?
  5. Would the project not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel?
  

Mike_Armstrong

Michael Armstrong, BS
Senior Professional Research Assistant

Michael is a graduate of the University of Northern Colorado with a Bachelor’s Degree in Biology with a Minor in Chemistry. Previous to working in the School of Pharmacy Mass Spectro​​metry Facility, Michael was a senior analytical chemist at Severn Trent/Core Laboratories for 9 years. His professional interests include mass spectrometry based metabolomics and analytical chemistry. He has over 20 years of mass spectrometry and analytical chemistry experienc​e.

Kat_Doenges

Katrina Doenges, MS
Senior Professional Research Assistant

Kat earned her MSc(Hons) in Environmental Chemistry from the University of Canterbury (Christchurch, New Zealand) and her BSc in Chemistry and Biochemistry from the University of California, Santa Cruz. She is skilled in various wet chemistry techniques and has some experience with ICP-MS and other instruments for detecting heavy metals and pesticides. Kat is new to metabolomics, but is quickly learning different sample preparation protocols, how to run the mass spec instruments and how to analyze data. She is looking forward to increasing her knowledge and abilities in these areas, as well as learning more about the administrative side of running a lab.​

Cole_Michel

Cole Michel, BS
Professional Research Assistant

Cole graduated from the University of Wisconsin – Madison with a bachelor’s degree in biochemistry. His professional interests are in proteomics, LC/MS method development, and utilizing analytical chemistry techniques for drug discovery. He has 6 months of mass spectrometry experience and 5 years of analytical chemistry experience.

Kevin_Quinn

Kevin Quinn, PhD
Post-Doctoral Researcher

Kevin obtained his B.S. in Chemistry from Saint John Fisher College and his Ph.D. from SUNY Buffalo where he gained experience in method development, and mass spectrometry instrumentation including LC-MS, and FTICR MS. For his current Post-doctoral work, Dr. Quinn uses mass spectrometry based assays to find perturbed metabolomics pathways in various biofluids to determine the underlying mechanisms associated with asthma.

Nichole_Reisdorph

Nichole Reisdorph, PhD
Associate Professor

Nichole received her MS in Biology and her PhD in Molecular Biology and Biochemistry at the University of South Dakota School of Medicine. She was trained in mass spectrometry and proteomic applications at the Scripps Research Center in La Jolla, CA and has extensive background in molecular biology with an emphasis on protein biochemistry techniques. Nichole is an Associate Professor in the Skaggs School of Pharmacy and she directs the UCD SOP Mass Spectrometry Core Facility.

Rick_Reisdorph

Richard Reisdorph, PhD
Associate Research Professor

Rick received his PhD in Molecular Biology and Biochemistry from the University of South Dakota School of Medicine and was a fellow with Peter Vogt at the Scripps Research Institute in La Jolla, CA. His work includes developing quantitative metabolomics and proteomics techniques and bioinformatics. He is currently the Assistant Director of the UCD SOP Mass Spectrometry Core Facility.

This tool allows interested potential postdoctoral candidates a centralized place to upload their basic information including CV and publications allowing them to e-mail it to up to three faculty member.

Postdoctoral Inquiries

  
A​​​gilent-6210-TOFAgilent 6210 TOF
Time-of-flight mass spectrometer equipped with Agilent 1290 UPLC and electrospray ionization (ESI). Typical uses for this instrument are metabolomics and small molecule analysis.
Agilent-6520-Q-TOFAgilent 6520 Q-TOF
A quadrupole-time-of-flight mass spectrometer equipped with Agilent 1290 UPLC and electrospray ionization (ESI). This instrument is typically used for proteomics, metabolomics (occasionally), and structural identification using MS/MS fragmentation.
Agilent-6550-quadrupoleAgilent 6550 quadrupole time-of-flight (Q-TOF) mass spectrometer
A quadrupole-time-of-flight mass spectrometer equipped with Agilent 1290 UPLC and electrospray ionization (ESI). The ESI source is equipped with Agilent iFunnel technology, resulting in higher sensitivity. Typical uses for this instrument are metabolomics, small molecule analysis, proteomics (occasionally), and structural identification using MS/MS fragmentation.
agilent-6560-IM-Q-TOFAgilent 6560 IM-Q-TOF
An ion mobility quadrupole-time-of-flight mass spectrometer equipped with Agilent 1290 UPLC and electrospray ionization (ESI). Typical uses for this instrument are metabolomics, small molecule analysis, and structural identification using MS/MS fragmentation. A unique feature of this mass spectrometer is the ability to separate ions based on their collision cross section. This adds an extra dimension of separation that occurs after chromatography and ionization.
Agilent-6410-QQQAgilent 6410 QQQ
A triple quadrupole mass spectrometer equipped with Agilent 1200 HPLC and electrospray ionization (ESI). This instrument is typically used for quantitative assays.
​Agilent-6490-QQQAgilent 6490 QQQ
A triple quadrupole mass spectrometer equipped with Agilent 1290 UPLC and electrospray ionization (ESI). The ESI source is equipped with Agilent iFunnel technology, resulting in higher sensitivity. This instrument is typically used for quantitative assays.
Bruker-EVOQ-QQQBruker EVOQ QQQ
A triple quadrupole mass spectrometer equipped with Bruker Advance UHPLC and electrospray ionization (ESI). This instrument is typically used for quantitative assays.
Bruker-Compact-Q-TOFBruker Compact Q-TOF
A quadrupole-time-of-flight mass spectrometer equipped with Dionex UltiMate 3000 UHPLC and electrospray ionization (ESI). This instrument is typically used for metabolomics, small molecule analysis, and structural identification using MS/MS fragmentation.
Bruker-impact-Q-TOFBruker Impact Q-TOF
A quadrupole-time-of-flight mass spectrometer equipped with Bruker NanoAdvance UHPLC and electrospray ionization (ESI). This instrument is typically used for proteomics.
Bruker-Scion-GC-MSBruker Scion GC-MS
A single quadrupole mass spectrometer equipped with gas chromatography and electron ionization (EI). This instrument is typically used for small molecule analysis. A unique feature of this instrument is the capability to do headspace GC.
Bruker-amaZon-ETDBruker amaZon ETD
A quadrupole ion trap mass spectrometer equipped with nanoflow liquid chromatography and electrospray ionization (ESI). This instrument is typically used for high sensitivity proteomics. Two fragmentation modes are offered with this instrument, electron transfer dissociation (ETD) and collision-induced dissociation (CID). ETD and CID fragment ions by different mechanisms, providing rich and complimentary structural information.
​ABI-4800-PlusABI 4800 Plus MALDI-TOF/TOF)
A time-of-flight/time-of-flight mass spectrometer equipped with matrix assisted laser desorption ionization (MALDI). This instrument is typically used for high sensitivity, high mass accuracy data in very short time frames, as well as MS/MS fragmentation.

​​Agilent Mass Hunter Workstation:

  • Qualitative Analysis
  • Quantitative Analysis
  • Profinder
  • Mass Profiler Professional (MPP)
  • PCDL Manager
  • Pathways to PCDL
  • DA Reprocessor ​

Bruker:

  • ProteinScape 3
  • Data Analysis 4.2
  • ProfileAnalysis 2.1​

Mass Spectrometry Users Meeting

Second Tuesday of the month from 11:30am - 1:00pm.

University of Colorado Denver – Anschutz Medical Campus
Skaggs School of Pharmacy and Pharmaceutical Sciences
12850 Montview Boulevard, Room V20-1108
Aurora, Colorado 80045

The Mass Spectrometry Users Meeting is intended to bring together all individuals that share interest in mass spectrometry-based research, for the purpose of sharing successes, failures, troubleshooting, idea generation, method development, networking, and collaboration opportunities. We have weekly presentations from lab members, collaborators, and external guests, followed by a brief question and answer session. The informal setting of these meetings encourages great discussions and breakthroughs.

Weekly email reminders for meetings are automatically sent out to the Department of Pharmaceutical Sciences. We also have a separate mailing list for affiliates and collaborators around the Denver-Boulder-Fort Collins area.

Please contact Roger Powell​ for more information.

The mass spectrometry facility offers a range of services for researchers doing proteomics, metabolomics, or small molecule research. We are continually working to add new services so please contact the facility directly if you do not see a specific service listed or if you are unsure about how to best utilize mass spectrometry in your research. If we are unable to provide the type of analysis you require we can often refer you to another facility that can.​

Our current prices can be located here​​.​ For services that have hourly rates, contact Cole Michel for pricing.​​

Assays Offered:​​

  • ​BCA protein assay
  • C18 clean up
  • Preomics
  • Acetylomics (Digest + IP)
  • Skin taping
  • In Gel Digest
  • In-Solution Digest FASP-Suspension
  • In-Solution Digest FASP- Dry
  • In-Solution Digest FASP-Immunodepletion
  • TFE In-Solution Digest
  • ID Gel
  • 11 cm 2D Gel
  • LC Proteomics
  • Accurate Mass Determination
  • HPLC Hourly (with labor)
  • HPLC Hourly (no labor)
  • Immunodepletion using MARS - Human Plasma
  • Immunodepletion using MARS - Human CSF
  • Immunodepletion using MARS - Mouse Plasma

Assays Offered:

  • Various Coverage of the Metabolome Depending on Sample Preparation (Click here for more information).
    • Methanol Protein Precipitation Extraction
  • Methanol Extraction is suitable for certain cellular extracts
  • Methanol extracts typically use one chromatographic method
    • Methyl tert-Butyl Ether (MTBE) Liquid-Liquid Extraction (LLE) (JOVE Publication)​.
  • Higher coverage can be obtained by separating the hydrophobic and hydrophilic compounds using LLE
  • Hydrophobic and hydrophilic fractions are analyzed using suitable chromatographic methods
  • Suitable for cell, biofluid and tissue samples
    • “Medium Throughput” MTBE Liquid-Liquid Extraction
  • Similar coverage as our traditional MTBE Liquid-Liquid Extraction Method, with higher daily sample prep throughput
  • Hydrophobic and hydrophilic fractions are analyzed using suitable chromatographic methods
  • Suitable for cell, biofluid and tissue samples
    • Urine Samples
  • Urine samples do not generally need to undergo an “extraction,” but do require some sample handling
  • Urine Samples typically use one chromatographic method.

​We also offer sample preparation training if you or a member of your lab would like to learn the protocol(s) suitable to process your own samples. Please contact Katrina Doenges for additional information.

Targeted Assays Powerpoint​​

  • Acylcarnitines (See Targeted List)
  • Amino Acids (See Targeted List)
  • Oxylipins "AKA Lipid Mediators" (See Targeted List)
  • Nucleotides Assay (See Targeted List)
  • Bile Acids
  • Endocannabinoids
  • Leukotriene E4 in Urine
  • Polycyclic Aromatic Hydrocarbons (PAHs)
  • Steroid Hormones
  • 25-OH Vitamin D2 and D3

Proteomics Hands-On Workshop

The Reisdorph Lab is offering Hands-On Workshops at the Skaggs School of Pharmacy on the University of Colorado Anschutz Medical Campus in Aurora, CO. Participants will learn introductory metabolomics or proteomics science and applicable protocols and technologies through a comprehensive, hands-on exploration of typical mass spectrometry-based workflows. Class size is limited to 10 participants to ensure excellent participant:instructor ratios. Workshops include topics such as experimental design, sample preparation, mass spectrometry basics, data acquisition, advanced technologies, and quantitative analyses. Over one-half of the workshop is spent in the laboratory performing sample preparation or acquiring/analyzing data on mass spectrometers. Several lectures and laboratories focus on data analysis strategies.

Please check back later for information on our next Proteomics Workshop.

Please e-mail Katrina Doenges or Elizabeth Sogunle with any questions.​​​​​​

 


 

Metabolomics Hands-On Workshop

The Reisdorph Lab offers a four-day Hands-On ​Metabolomics Workshop​​ at the Skaggs School of Pharmacy on the Anschutz Medical Campus in Aurora, CO. Participants will learn introductory metabolomics science and applicable protocols and technologies through a comprehensive, hands-on exploration of typical mass spectrometry-based workflows.

Please check back later for information on our Metabolomics Workshop.

For more information please e-mail Katrina Doenges or Elizabeth Sogunle.


​Below please find our Master Service Agreement template approved by the University's legal department. All outside CU collaborators must have a fully executed MSA prior to our CORE receiving samples and/or performing work.​

Please have the signing authority and their respective legal department review and execute. Be aware, the PI's are not authorized to sign on behalf of their Universities, typically. Additionally, a Statement of Work must accompany any new project outside the original MSA, regardless of previous collaboration. Once reviewed and signed, please send this document to David Stein​. If there are any questions or concerns, please respond to the same e-mail.​