Dr. Jed Lampe is an R01-funded principal investigator, educator, and scientific leader in the ADME/DMPK field. After receiving his Ph.D. in Medicinal Chemistry from the University of Washington, he completed post-doctoral training at UCSF under the tutelage of Professor Paul Ortiz de Montellano, where he studied ligand binding dynamics and their relationship to enzyme function in cytochrome P450 enzymes using pioneering 2-dimensional NMR techniques with site-specifically incorporated unnatural amino acids. Currently, he serves as an Assistant Professor in the Department of Pharmaceutical Sciences at the University of Colorado, Skaggs School of Pharmacy where his research interests involve the application of bioanalytical, biophysical, and computational techniques to understand and predict drug metabolism and disposition in special populations, including the neonate and developing infant. He serves on a number of editorial boards, including: Nature Scientific Reports, Current Drug Metabolism, and PLOS One.
Education, Licensure & Certifications
- Post-doctoral Fellowship: Pharmaceutical Chemistry, University of California, San Francisco
- PhD: Medicinal Chemistry, University of Washington
- MS: Microbiology, Idaho State University
- BS: Biological Sciences, Walla Walla University
- 2020 University of Washington School of Pharmacy “125 Legendary Alumni” Award
- 2010-2011, The K-INBRE Faculty Recruitment Award
- 2009-2010, UCSF Post-doctoral Scholar Research Award
- 2005-2006 Merck Graduate Student Scholar Award
- 2002-2005, NIH Pharmacological Sciences Training Grant Pre-doctoral Fellow
- Member, American Chemical Society
- Member, International Society for the Study of Xenobiotics
- Member, American Society for Pharmacology and Experimental Therapeutics
- Editorial Board Member, PLOS One
- Editorial Board Member, Current Drug Metabolism
- Editorial Board Member, Nature Scientific Reports
- Member, Society of Toxicology
- Member, American Association of Colleges of Pharmacy
- Member, Sigma Xi Scientific Honor Society
- Member, Biophysical Society
- cytochrome P450 enzymes
- drug metabolism and disposition
- Structural and Functional Modeling of HIV Drug Metabolism in the Infant Liver: Using both in vitro methods and novel iPS cell culture approaches, we are obtaining the kinetic (Km, Vmax, CLint) and structural (X-ray crystal structure) parameters that define metabolism of HIV inhibitors by CYP3A7, a neonatal specific CYP isoform.
- Computational Modeling of HIV Drug Metabolism and Transport in the Infant Liver: In order to be able to accurately predict drug metabolism and disposition in the developing infant, we need to have reliable computational tools. To this end, data obtained from the in vitro and cell culture studies is being modeled to obtain the QSAR of CYP3A7 mediated metabolism of HIV inhibitors, and also to build a PBPK model of their disposition using the Simcyp™ software package.
- Development of Micro-organoid Models of the Infant Liver: Creating a 3D cellular microenvironment that accurately mimics the functions of the infant liver is of vital importance to preserve endogenous functions in a relevant model system. Although the liver has been especially challenging due to its notable cellular heterogeneity, the “organ-on-a-chip” technologies have shown promise in mimicking these cellular microenvironments. Currently, the Lampe lab is in the early stages of developing a micro-organoid model of the infant liver that would be compatible with the “organ-on-a-chip” technology that has been exploited with other organ types.
Publications and Presentations
Kandel, S.E., Lampe, J.N. (2021). Inhibition of CYP3A7 DHEA-S oxidation by Lopinavir and Ritonavir: an alternative mechanism for adrenal impairment in HIV antiretroviral treated neonates. Chem. Res. Tox. In press. E-print: https://pubs.acs.org/doi/pdf/10.1021/acs.chemrestox.1c00028
Li, H., Lampe, J.N. (2019). Neonatal cytochrome P450 CYP3A7: A comprehensive review of its role in development, disease, and xenobiotic metabolism. Arch Biochem Biophys. 673:108-118. PMID: 31445893.
Bishop, S.C., Winefield, R., Anbanandam, A., Lampe, J.N. (2019). Aqueous synthesis of a small-molecule lanthanide chelator amenable to copper-free click chemistry. PLoS One. 14(3): 209-226, PMID: 30917122.
Boxberger, K.H., Hagenbuch, B., Lampe, J.N. (2018). Ligand-dependent modulation of OCT1 transport by cationic substrates and the negatively charged anion, BSP. Biochem. Pharmacol. 156: 371-384, PMID: 30138624.
Kandel, S.E., Han, L.W., Mao, Q., Lampe, J.N. (2017). Digging deeper into CYP3A testosterone metabolism: kinetic, regio- and stereoselectivity differences between CYP3A4/5 and CYP3A7. Drug Metab. Dispo. 45: 1266-1275, PMID: 28986474.
Lampe, JN. (2017). Emerging biophysical techniques to monitor protein-protein interaction in drug metabolizing enzymes. Front. Pharmacol. 8: 521-534, PMID: 28848438.