Our work is focused in two main areas. First, we are interested in the molecular mechanisms of transcriptional regulation using the nuclear receptor superfamily as a model system. We use a blend of biophysical, structural and cell biological approaches to dissect the various protein-DNA and protein-protein interactions critical to transcriptional activation, and then use this information to synthesize and predict overall behavior. Our second focus is on characterizing the molecular interactions of therapeutic proteins, especially at high concentrations and under formulation/delivery conditions. This work is primarily directed toward monoclonal antibodies, but we are also interested in therapeutic peptides and vaccines.
Education, Licensure & Certifications
- BA, University of Vermont
- PhD, Johns Hopkins University
- Post-doctoral, University of Colorado
- Pharmaceutical Sciences Program
- Biomolecular Structure Program
- Reproductive Sciences Program
- Medical Scientist Training Program
- Biomedical Sciences Program
- Biophysical characterization of therapeutic macromolecules
- Mechanisms of transcriptional regulation
- Thermodynamics of protein-protein and protein-DNA interactions
Publications and Presentations
Hopkins MM, Lambert CL, Bee JS, Parupudi A, Bain DL (2018) Determination of interaction parameters for reversibly self-associating antibodies: A comparative analysis. J Pharm Sci, 107, 1820-1830.
De Angelis, RW, Maluf, NK, Yang, Q, Lambert, JR, and Bain, DL (2015) Glucocorticoid receptor dissociation kinetics measured in vitro reveal exchange on the second time scale. Biochemistry, 54, 5306-5314.
Bain DL, Yang Q, Connaghan KD, Robblee JP, Miura MT, Degala GD, Lambert JR, Maluf NK. (2012) Glucocorticoid receptor-DNA interactions: Binding energetics are the primary determinant of sequence-specific transcriptional activity. J Mol Biol, 422, 18-32