Mass Spectrometry Facility

Aut​​horship

Co-authorship is generally expected when core personnel have made significant contributions to the research in the form of consultation, experimental design, method development, data analysis and/or data interpretation. Significant is defined as “the project would not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel”. In such cases, core personnel should have the opportunity to review and edit the appropriate sections of a manuscript before submission.

Acknowledgement of facility contributions is expected in publications that include any data generated in the facility. For example, when fee-for-service is performed with no method development and minimum effort by Core personnel. An example of an appropriate acknowledgment is “The authors wish to thank the University of Colorado School of Pharmacy, Mass Spectrometry Facility for analyzing samples”.

Please inform us when relevant publications are accepted and forward us a copy for our records. We may also post citation information on our website. This information is vital to the continued support of the facility.

Grant Assista​nce

We are very happy to provide letters of support and assistance in grant writing. For letters of support, please allow at least 2 weeks from the time of your request. Please furnish the following information: Title of grant, agency to which you are submitting the grant, your title and mailing address, and 1-3 sentences on the goals (or specific aims) of your proposal.

Our facility is also dependent on grant support for long-term success and we appreciate your support in keeping the facility funded. To determine if percent effort by core personnel is required, please consider the following:

1. Does the proposal include a specific aim(s) that focuses on mass spectrometry?
2. Is method development is required?
3. Are there large numbers of samples are to be ​analyzed?
4. Would the aim or proposal not receive a favorable review without the support of Core personnel?
5. Would the project not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel?

Vendors

• Bruker
• Agilent
• Sciex

Databases

• Metlin
• HMDB
• KEGG
• BIOCYC
• Lipid Maps

Other Resources

• COSMOS - COordination of Standards in MetabOlomics
• Metabolomics Society
• NIH Common Fund Metabolomics Program
• Protein Prospector
• Ion Source
• Colorado Biological Mass Spectrometry Society - CBMSS
• American Society for Mass Spectrometry - ASMS

This tool allows interested potential postdoctoral candidates a centralized place to upload their basic information including CV and publications allowing them to e-mail it to up to three faculty member.

Postdoctoral Inquiries

The mass spectrometry facility offers a range of services for researchers doing proteomics, metabolomics, or small molecule research. We are continually working to add new services so please contact the facility directly if you do not see a specific service listed or if you are unsure about how to best utilize mass spectrometry in your research. If we are unable to provide the type of analysis you require we can often refer you to another facility that can.​

Our current prices can be located here​​.​ For services that have hourly rates, contact Cole Michel for pricing.​​

Assays Offered:​​

• ​BCA protein assay
• C18 clean up
• Preomics
• Acetylomics (Digest + IP)
• Skin taping
• In Gel Digest
• In-Solution Digest FASP-Suspension
• In-Solution Digest FASP- Dry
• In-Solution Digest FASP-Immunodepletion
• TFE In-Solution Digest
• ID Gel
• 11 cm 2D Gel
• LC Proteomics
• Accurate Mass Determination
• HPLC Hourly (with labor)
• HPLC Hourly (no labor)
• Immunodepletion using MARS - Human Plasma
• Immunodepletion using MARS - Human CSF
• Immunodepletion using MARS - Mouse Plasma

Assays Offered:

• Various Coverage of the Metabolome Depending on Sample Preparation (Click here for more information).
  • Methanol Protein Precipitation Extraction
• Methanol Extraction is suitable for certain cellular extracts
• Methanol extracts typically use one chromatographic method
  • Methyl tert-Butyl Ether (MTBE) Liquid-Liquid Extraction (LLE) (JOVE Publication)​.
• Higher coverage can be obtained by separating the hydrophobic and hydrophilic compounds using LLE
• Hydrophobic and hydrophilic fractions are analyzed using suitable chromatographic methods
• Suitable for cell, biofluid and tissue samples
• Similar coverage as our traditional MTBE Liquid-Liquid Extraction Method, with higher daily sample prep throughput
• Hydrophobic and hydrophilic fractions are analyzed using suitable chromatographic methods
• Suitable for cell, biofluid and tissue samples
  • Urine Samples
• Urine samples do not generally need to undergo an “extraction,” but do require some sample handling
• Urine Samples typically use one chromatographic method.

​We also offer sample preparation training if you or a member of your lab would like to learn the protocol(s) suitable to process your own samples. Please contact Katrina Doenges for additional information.

Targeted Assays Powerpoint​​

• Acylcarnitines (See Targeted List)
• Amino Acids (See Targeted List)
• Oxylipins "AKA Lipid Mediators" (See Targeted List)
• Nucleotides Assay (See Targeted List)
• Bile Acids (See Targeted List)
• Endocannabinoids (See Targeted List)
• Leukotriene E4 in Urine
• Polycyclic Aromatic Hydrocarbons (PAHs)
• Steroid Hormones
• 25-OH Vitamin D2 and D3
• Fatty Acid Methyl Esters (FAMES) (See Targeted List)
• TCA cycle (See Targeted List)

Metabolomics and Proteomics Hands-On Workshops

The Reisdorph Lab is offering Hands-On Workshops at the Skaggs School of Pharmacy on the University of Colorado Anschutz Medical Campus in Aurora, CO. Participants will learn introductory metabolomics or proteomics science and applicable protocols and technologies through a comprehensive, hands-on exploration of typical mass spectrometry-based workflows. Class size is limited to 10 participants to ensure excellent participant to instructor ratios. Workshops include topics such as experimental design, sample preparation, mass spectrometry basics, data acquisition, advanced technologies, and quantitative analyses. Over one-half of the workshop is spent in the laboratory, performing sample preparation and acquiring/analyzing data on mass spectrometers. Several lectures and laboratories focus on data analysis strategies.

Please check back later for information on upcoming Metabolomics and Proteomics Workshops.

For more information please e-mail Katrina Doenges.

​Below please find our Master Service Agreement template approved by the University's legal department. All outside CU collaborators must have a fully executed MSA prior to our CORE receiving samples and/or performing work.​

Please have the signing authority and their respective legal department review and execute. Be aware, the PI's are not authorized to sign on behalf of their Universities, typically. Additionally, a Statement of Work must accompany any new project outside the original MSA, regardless of previous collaboration. Once reviewed and signed, please send this document to your Mass Spec Core contact person. If there are any questions or concerns, please respond to that person as well.

​​Agilent Mass Hunter Workstation:

• Qualitative Analysis
• Quantitative Analysis
• Profinder
• Mass Profiler Professional (MPP)
• PCDL Manager
• Pathways to PCDL
• DA Reprocessor ​

Bruker:

• ProteinScape 3
• Data Analysis 4.2
• ProfileAnalysis 2.1​